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KMID : 0985420110330010031
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Laboratory Medicine and Quality Assurance
2011 Volume.33 No. 1 p.31 ~ p.37
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Detection of Varicella-Zoster Virus (VZV) by Nested Polymerase Chain Reaction
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Lee Hyoung-Nam
Kim Tae-Eun
Bark Hyeon-ju
Lee Hyun-Im
Jung Eun-Sun
Choi Yeong-Jin
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Abstract
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BackgroundPolymerase chain reaction (PCR) is known as a sensitive and specific method for the detection of varicella-zoster virus (VZV). Nested PCR is reliably used than conventional PCR to increase the sensitivity and specificity, especially in cases of small sized tissue samples.
Methods:We detected VZV infection in tissues from 111 patients using conventional PCR and nested PCR. Ninety-one cases of fresh tissues and twenty cases of formalin-fixed paraffin-embedded (FFPE) tissues were evaluated. The column method or home made lysis buffer method was used for the DNA extraction of fresh tissues and FFPE tissues.
Results:Among total 111 cases, VZV were detected in 62 (55.9%) cases by conventional PCR and 79 (71.2%) cases by nested PCR. The detection rate of nested PCR was higher than conventional PCR (1.27 folds). In 91 cases of fresh tissues, 56 (61.5%) were positive by conventional PCR and 68 (74.7%) by nested PCR. In 20 cases of FFPE tissues, 6 (30%) were positive by conventional PCR and 11 (55%) by nested PCR. The detection rate of VZV was increased by nested PCR both in fresh tissues (1.21 folds) and FFPE tissues (1.83 folds).
Conclusion:Nested PCR is the more sensitive method than conventional PCR for the detection of VZV infection in tissues regardless of DNA extraction methods, especially for the small sized FFPE tissues.
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KEYWORD
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Varicella-zoster virus (human herpesvirus 3), Nested polymerase chain reaction, Formalin-fixed paraffin-embedding (FFPE)
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